Great CD45RA Human Antibody from BioLegend

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-human CD45RA BV605

Catalog Number:

304134

Clone Number:

In our work, we focus on the study of T cells in various human organs. In order to properly subset the T cells, we need reliable antibodies and so we chose this one because it is bright and works very well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human lung cells

Primary Incubation

Cells were stained in 100ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

Using this antibody we could clearly identify the CD45RA positive and negative populations in our sample.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This antibody is a great choice for CD45RA in humans.

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