Great Antibody for Human CCR7 from BioLegend

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-human CCR7 AF488

Catalog Number:

353206

Clone Number:

Our lab focuses our work on the study of T cells in humans organs across different age groups. In order to study the different types of T cells, we need reliable markers to identify the different subsets. We chose this antibody because it is bright and works well to identify CCR7+ cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human lung cells

Primary Incubation

Cells were stained in 100ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

Using this antibody we were able to assess CCR7 expression on our population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This antibody works well for human CCR7+ cells.

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