Great Human CD69 Antibody from BD Biosciences

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Anti-human CD69 BUV395

Catalog Number:

564364

Clone Number:

Browse Similar Products: CD69 Flow Cytometry Antibodies

Our lab focuses our study on T cells in human organs. In order to study differences in T cells throughout the body and at different ages, we need reliable T cell marker antibodies. We chose this antibody for CD69 because it is bright and does not spill into other channels.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human lung cells

Primary Incubation

Cells were stained in 100ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

This antibody worked very well to show the expression of CD69 on our target cell population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This antibody is a great choice for human CD69 expression.

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