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Alexa Fluor 647 anti-human/mouse Granzyme B Antibody
515406
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Our lab studies the mTOR signaling pathway in T cell differentiation with an emphasis on both CD4 and CD8 T cells. We routinely conduct flow cytometry experiments to assess effector function based on lytic molecules such as granzyme B.We are looking at the intracellular staining of mouse granzyme B in activated CD8 T cells when stimulated with antigen
Flow Cytometry
Activated murine CD8+ T cells
1:500 in BD Permwash buffer (Intracellular staining)
Surface stain with FC block
None
BD FACS Celesta
We are easily able to identify granzyme B positive CD8+ T cells.
N/A
Very robust antibody to detect.
Reliable antibody to detect granzyme B in T cells. Took a long time to acquire a good antibody and clone. Very satisfied.