Time Course Effect of Chemoattractant on VEGF1 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

VEGF1

Catalog Number:

sc-7269

Clone Number:

VEGF, originally known as vascular permeability factor (VPF), it is a signal protein produced by cells that stimulates the formation of blood vessels. VEGF is a potent angiogenic factor and was first described as an essential growth factor for vascular endothelial cells. Here we checked the time course effect of VEGF expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 dilution in 2% skim milk prepared in 1X TBST

Blocking Agent

2% skim milk prepared in 1X TBST

Secondary Incubation

1:4000 2% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of VEGF expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody is highly specific and works great for western blot.

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