Time Course Effect of Chemoattractant on VDUP1 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology

Product Name:

VDUP1

Catalog Number:

sc-271238

Clone Number:

VDUP1 is regulated in response to various stresses, including Ros, UV and heat shock. The transcription factor HSF may be involved in this regulation. It may act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Here we checked the time course effect of VDUP1 by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 dilution in 2% skim milk prepared in 1X TBST

Blocking Agent

2% skim milk prepared in 1X TBST

Secondary Incubation

1:4000 2% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found VDUP1 expression was significantly inhibited by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody works great for western blot. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion