Time Course Effect of Chemoattractant on Sod1 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

Sod1

Catalog Number:

sc-11407

Browse Similar Products: SOD-1 Western Blot Antibodies

SOD1 is an enzyme that is encoded in human by the SOD1 gene. SOD1 is one among three human superoxide dismutases. It involved in apoptosis and familial amyotrophic lateral sclerosis. Here we checked the time course effect of chemoattractant on Sod1 expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:2000 dilution in 2% skim milk prepared in 1X TBST

Blocking Agent

2% skim milk prepared in 1X TBST

Secondary Incubation

1:4000 2% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

There is a significant increase in Sod1 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody detects the Sod1 specifically. It works great for western blot.

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