Time Course Effect of Chemoattractant on Trx1 Expression

May 03, 2019

BlueskyReddit
Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology

Product Name:

Trx1

Catalog Number:

sc-20146

Clone Number:

Browse Similar Products: Trx1 Western Blot Antibodies

Thioredoxin is a small class of proteins present in Prokaryotes and Eukaryotes. It plays a important role in many biological processes, including redox signaling. Here we checked the time course effect of Trx by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 dilution in 2% skim milk prepared in 1X TBST

Blocking Agent

2% skim milk prepared in 1X TBST

Secondary Incubation

1:4000 2% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of Trx1 by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody is very specific and the signal is strong. Highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion