eBioscience Thermo Fisher
IFN gamma Monoclonal Antibody APC
17-7311-82
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Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. T cell differentiation requires a dynamic change in transcriptional activity leading to effector programming. To assess effector function of CD8 T cells, we measure IFNg cytokine expression through flow cytometry.
Flow Cytometry
Activated murine CD8+ T cells
1:1000 in 1x BD Permwash buffer (Intracellular Staining)
Surface stain with FC Block before Fix/Perm (BD CytoFix/Perm)
None
BD FACS Celesta
Upon T cell stimulation, we are easily able to detect CD8+IFNg+ cells using flow cytometry.
N/A
Titrate to ensure not too bright.
Very robust and bright antibody at very good dilution makes it cheap in the long run.
None.
Great antibody to assess effector function of T cells based on IFNg secretion.