Sigma
Anti-β-Tubulin Antibody
05-661
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Browse Similar Products: Beta-Tubulin Immunofluorescence Antibodies
Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. mTOR signaling controls the metabolic programming of T cells. We wanted to determine segregation of proteins in daughter cells; therefore, we use a tubulin bridge as a means to identify dividing cells.
Immunofluorescence
Murine CD8+ T cells
1:1000 in PBS/.2%Tween
10% Goat Serum
1:500 anti-mouse AF405
None
Zeiss 780 Confocal Micro
We are able to identify dividing CD8 T cells based on the tubulin bridge through confocal microscopy.
27064374
Make sure you perform staining with any rat antibodies before mouse otherwise there is cross-reactivity between secondary anti-mouse to rat antibodies
Reliable and robust antibody
None.
Highly recommend this antibody if you need a mouse isotype anti-tubulin antibody for confocal microscopy.