Automated Cell Counter without Consumables

May 01, 2019

BlueskyReddit
Johns Hopkins School of Medicine
Oncology
Graduate Student

Overall

Performance

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Countess™ II Automated Cell Counter

Catalog Number:

AMQAX1000

Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. mTOR signaling controls the metabolic programming of T cells. Programming of T cells also involves dynamic changes in mitochondria mass and morphology. We routinely count primary T cells (murine and human) using a traditional hemacytometer. We wanted to use this automated counter to help with the process and make it faster.

Experimental Design and Results Summary

Application

Counting Cells

Tips

Does not count T cells very well.

Features Summary

DOI or PMID #

N/A

Image Gallery

Summary

The Good

No consumables are involved since re-usable slide

The Bad

Can not accurately count T cells

The Bottom Line

This instrument performs quite well for big cells like tumor cells but it can not distinguish viable or non viable T cells due to size. Therefore, this machine doesn't do well with small cells.

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