Cell Signaling Technology
Phospho-Btk (Tyr223) (D9T6H) Rabbit mAb #87141
87141
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In our lab, we aim to study a small molecule kinase inhibitor’s activities in various cell lines by investigating phosphor-proteins of interest expression levels. This antibody that we used is good and reliable and worked without much optimization. Showed consistent results.
Western Blot
Ramos cell lysates
1:1000, overnight, four degrees Celsius
Licor blocking solution
1:5000, 2 hours, room temp
None
Fluorescence imaging
The Ramos cells were treated with IgM stimulation (10ug/mL) for 10 minutes, and p-BTK Y223 signal increased as expected. This is a good antibody to use in Western blot.
N/A
Easy to use, reliable results
This is a good antibody to use in Western blot.