Good Anti-ß-Actin (13E5) from CST

Gastroenterology
Charite University Hospital
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Anti-β-actin (13E5)

Catalog Number:

4970

Browse Similar Products: Beta-Actin Western Blot Antibodies

This β-Actin antibody should detect endogenous levels of total β-actin protein and is used as a control in western-blotting. We used this antibody as a control for our Western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Epithelial cells

Primary Incubation

1:1000, overnight, 4°C

Blocking Agent

5 % BSA in TBST

Secondary Incubation

1:1000, 2 hrs, RT

Tertiary Incubation

1:1, 5 mins, RT

Detection

Fujiflm Las 3000

Results Summary

Had bright and even bands. High Resolution

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright bands, good control for WB

The Bad

N/A

The Bottom Line

I would recommend this antibody for WB

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