Anti-IL-17a A488

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 488 anti-human IL-17A Antibody

Catalog Number:

512307

We used this anti-IL-17a antibody to detect IL-17a secretion in innate lymphocytes after Stimulation with various signals.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cultured cells

Primary Incubation

15min, 37c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD Facs Fortessa

Results Summary

After Stimulation, IL-17a could be detected; however, it was very dull and the resolution was low. This antibody also did not detect IL-7F and thus, was specific.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific

The Bad

Dull, low resolution

The Bottom Line

Okay antibody. Would try in a different fluorochrome.

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