Good Antibody for CD5 in Mouse from BD Biosciences

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COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Anti-mouse CD5 BUV737

Catalog Number:

612809

Clone Number:

Browse Similar Products: CD5 Flow Cytometry Antibodies

Our lab has a study of T cell involved in a mouse influenza infection model. One of our determined targets is CD5 and we wanted an antibody that was bright and did not spill over into other channels.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung cells

Primary Incubation

Cells were stained in 100ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

We were able to clearly see how CD5 expression shifts in different cell experiments using this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This antibody works well in mouse T cells.

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