Bright TCF1 Antibody for Human and Mouse from CST

April 18, 2019

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signalling Technologies

Product Name:

Anti-mouse/human TCF1 PE

Catalog Number:

14456

Clone Number:

One component of our studies in immunology is the activation of T cells from both mouse and human. One of our targets is TCF1 and we chose this antibody because it can work in both species.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung cells

Primary Incubation

Cells were fixed in Tonbo fix/perm buffer and then stained at 1:50 in perm buffer for 30 minutes at room temperature.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

We are able to track the expression of TCF1 in both human and mouse cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This antibody works well for human and mouse T cell staining.

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