Easy-to-Use CD25 Antibody from BioLegend

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-mouse CD25 BV650

Catalog Number:

102038

Clone Number:

In our lab we are studying the role of T cells in a mouse influenza infection model. We are interested in locating and studying activated T cells and thus use CD25 as a marker for this. We chose this antibody because it is very bright.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung cells

Primary Incubation

Cells were stained in 100ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

We we able to clearly identify CD25+ T cells for future analysis.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

Great antibody for CD25 staining on mouse lymphocytes.

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