Great PCR Master Mix for Next-Generation Sequencing Sample Prep

Harvard Medical School
Cell Biology
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

NEBNext® Ultra™ II Q5® Master Mix

Catalog Number:

M0544

We use this Q5 master mix to prepare samples for Illumina Miseq sequencing. This reagent is supposed to be highly efficient and ultra-high fidelity, so it is crucial for successful sequencing sample prep.

Experimental Design and Results Summary

Application

Next generation sequencing sample prep

Starting Material

N/A

Tips

For genotyping PCR using this kit, I use 10 ul reaction per sample to save reagent

Results Summary

Always generates accurate sequencing results.

DOI or PMID #

N/A

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Summary

The Good

Reliable, high quality, reproducible results

The Bad

Very expensive

The Bottom Line

Good choice of reagent to prepare next generation sequencing samples.

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