The Kit to Make Guide RNA for CRISPR Gene Editing

April 19, 2019

BlueskyReddit
Harvard Medical School
Cell Biology
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen by Thermo Fisher Scientific

Product Name:

GeneArt Precision gRNA synthesis kit

Catalog Number:

A29377

This kit is very useful to make guide RNA for CRISPR experiments. You just need to order the oligos for the designed guide RNA, and follow the protocols to make guide RNA which is much cheaper than buying from companies.

Experimental Design and Results Summary

Application

Making guide RNA for CRISPR

Starting Material

N/A

Tips

Follow the protocol

Results Summary

Can generate high-quality guide RNA which I confirmed by running agarose gel and concentration measurement. The CRISPR experiments using those guide RNAs also worked very well.

DOI or PMID #

N/A

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Summary

The Good

Cheap and high quality

The Bad

More work than ordering from a company

The Bottom Line

It's a very reliable kit to make guide RNA at home instead of spending four times more money ordering from companies. It can also be faster than buying from a company.

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