Thermo Fisher Scientific (Invitrogen)
eBioscience™ Permeabilization Buffer (10X)
00-8333-56
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I use this buffer to permeabilize cell samples for staining of intracellular antigens, and it worked well. Cell samples prepared using this reagent always generates good signal based on flow cytometry analysis.
Flow cytometry sample prep
N/A
Dilute 10 fold to make 1x working solution, keep the stock at 4C
When staining proteins like transcription factors which are located inside nucleus, if permeabilization is not efficient, the staining does not work or the wash is not complete. This permeabilization solution can help to avoid such problem
Easy to use, reliable
None
This is a good choice of permeabilization buffer if you are staining proteins inside the nucleus or other organelles.