MilliporeSigma
Betaine solution
B0300
I always use betaine in my PCR reaction for genotyping which greatly helps to get rid of non-specific bands. I had many cases where adding betaine makes a huge difference in the PCR result.
PCR for genotyping
N/A
Use 10% betaine in the reaction mix
By adding betaine in the PCR reaction, my genotyping PCR is much more specific and generates very neat results compared to without betaine addition
Greatly improve results
None
I highly recommend trying betaine in your PCR reaction if you have a problem with getting a specific band.