Hygromycin B Solution in PBS (50mg/mL) for Selection

Harvard Medical School
Cell Biology
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Hygromycin B

Catalog Number:

10687010

This is the ready-to-use hygromycin B solution for selection when generating a cell line that has hygromycin resistence. The solution is sterile and can be added directly to cultured cells to start selection.

Experimental Design and Results Summary

Application

Cell selection

Starting Material

Cultured mammalian cells

Tips

Different concentration should be tested for different cells

Results Summary

I use this hygromycin B solution to select for positive clones when doing CRISPR gene editing. The selected clones were usually successful in editing.

DOI or PMID #

N/A

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Summary

The Good

Convenient, easy to use

The Bad

A little more expensive.

The Bottom Line

I would recommend using this hygromycin B solution if you need to use this selection method for the cell engineering project.

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