Thymidine for Cell Cycle Synchronization

Harvard Medical School
Cell Biology
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Thymidine

Catalog Number:

T1895-5G

I use this thymidine to make thymidine solutions for double thymidine block to synchronize cells for cell cycle analysis.

Experimental Design and Results Summary

Application

Cell synchronization

Starting Material

Mammalian cell

Tips

Make 100 mM stock and use 2 mM final concentration

Results Summary

Around 90% of the cells were successfully synchronized at G1/S stage

DOI or PMID #

N/A

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Summary

The Good

High purity chemical

The Bad

Need to make the solution by yourself

The Bottom Line

This is a high quality thymidine reagent that generates good results for cell cycle related experiments. Not toxic to the cells.

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