Excellent Reagent for Glycoprotein Purification

Case Western Reserve University
Ophthalmology
research associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Vector laboratories

Product Name:

Agarose bound Lotus Tetragonolobus Lectin (LTL)

Catalog Number:

AL-1323-2

I have used this lectin to purify a glycoprotein with fucose and could obtain the good purified one. It worked very well.

Experimental Design and Results Summary

Application

Glycoprotein purification using agarose-conjugated LTL

Starting Material

N/A

Protocol Overview

Tips

I had to use binding bfr with Ca2+ and elution buffer which was purchased from the company.

Results Summary

This lectin has worked very well, by which I could purify a glycoprotein with fucose or withough fucose.

DOI or PMID #

N/A

Additional Notes

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Summary

The Good

Good purification

The Bad

it is not easy to expect the purification capacity by this lectin

The Bottom Line

This is very good. I will use this again.

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