Iba1 Antibody for Immunofluorescence

April 09, 2019

Immunology
MSKCC
Research Fellow

Overall

Quality of Results

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Company:

Wako

Product Name:

Anti Iba1

Catalog Number:

019-19741

In an effort to find additional markers to stain macrophages in mammary gland, we stained for iba1

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed mouse (C57BL/6) lactating mammary gland cryosections

Primary Incubation

1 in 200

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum, 2% goat serum

Secondary Incubation

Goat anti-rabbit Alexa Fluor 647 (Thermofisher, A-21245) 1:500

Tertiary Incubation

None

Detection

Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Very good staining of macrophages: Iba1 (green), F4/80 (red) & Hoechst (blue)

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable & reproducible staining of Iba1+ cells

The Bad

None

The Bottom Line

As expected, excellent Iba1 staining.

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