Bio-Rad
Alexa Fluor® 488 anti-mouse CD206 Antibody
MCA2235A488
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In our effort to understand mammary gland tumorgenesis, we stained mammary gland cryosections from young (10 weeks old) PyMT mice for the macrophages markers: mannose receptor (CD206) & F4/80
Immunofluorescence
PLP fixed mouse (C57BL/6) PyMT mammary gland cryosections
1 in 200
1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum
None
Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.
Good staining for CD206+ cells: CD206 (green), F4/80 (red) & Hoechst (blue)
N/A
Reliable & reproducible staining of CD206+ cells in mammary gland
Good antibody for direct staining of CD206 epitope.