Granzyme B Antibody for Immunofluorescence

March 11, 2019

Immunology
MSKCC
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-human/mouse Granzyme B Antibody

Catalog Number:

515406

Clone Number:

We study tumorgenesis in PyMT model. We stained early transformed (8 weeks old) PyMT mice for CD3 and Granzyme B in order to locate the Granzyme B+ lymphocytes.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed mouse (C57BL/6) PyMT mammary gland cryosections

Primary Incubation

1 in 100

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Good staining of Granzyme B+ cells: CD3 (green), Granzyme B (red), Hoechst (blue).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable & reproducible staining of Granzyme B+ cells

The Bad

Its seems to stain well only the Granzyme Bhigh cells

The Bottom Line

Good antibody for Granzyme B staining.

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