Easy-to-Use Kit for Interrogating Mouse Serum Insulin Levels

April 08, 2019

BlueskyReddit
Johns Hopkins University
Oncology
Postdoctoral Researcher

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Company:

MilliporeSigma

Product Name:

Rat/Mouse Insulin ELISA

Catalog Number:

EZRMI-13K

My research focuses on the role of the immune system in regulating blood glucose. In this experiment, Wild-type C57B6 mice (Wt) were started on a high fat diet at 6 weeks of age and maintained on the diet for 3 months. Then blood was harvested from the mice and serum isolated. Serum insulin levels were evaluated using this kit.

Experimental Design and Results Summary

Application

ELISA on mouse serum

Starting Material

Mouse serum

Protocol Overview

Tips

There are many steps so make sure you keep track of which step you are on

Results Summary

In this experiment, Wild-type C57B6 mice (Wt) were started on a high fat diet at 6 weeks of age and maintained on the diet for 3 months. Then blood was harvested from the mice and serum isolated. Serum insulin levels were evaluated using this kit. As expected, high fat diet induced insulin resistance and this was indicated by an increase in serum insulin levels.

DOI or PMID #

N/A

Additional Notes

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Summary

The Good

Easy to use with precoated plates

The Bad

It is expensive; uses pre-coated plates which are nice, but this increases the price

The Bottom Line

This is a great easy kit for evaluating insulin levels in mouse serum. It is easy to use with pre-coated plates. However the pre-coated plate convenience increases the cost. The kit includes positive controls which gives me more confidence in the results.

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