Good Antibody for Evaluating VDAC Expression

April 03, 2019

BlueskyReddit
Oncology
Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

VDAC Rabbit mAb

Catalog Number:

4661

My research focuses on the role of the immune system in regulating blood glucose. In this experiment mouse skeletal muscle was lysed and ran out on a gel. VDAC was detected in a single band at the correct size.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Skeletal Muscle

Primary Incubation

4% BSA in TBST overnight at 4*C

Blocking Agent

5% milk in TBST

Secondary Incubation

5% milk in TBST for 2 hours at room temperature

Tertiary Incubation

N/A

Detection

ECL

Results Summary

In this experiment mouse skeletal muscle was lysed and ran out on a gel. VDAC was detected in a single band at the correct size.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Gives a single band at the correct size

The Bad

Can give a weak signal depending on the sample

The Bottom Line

This is a good antibody that gives a nice clean band at the correct size. The only drawback is that depending on the sample type, it can give a weak signal.

Share your experience with other scientists. Write a Review! »

Join the discussion