Reliable Antibody for Looking at FNDC5

Oncology
Johns Hopkins University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-FNDC5 antibody

Catalog Number:

ab174833

My research focuses on the role of the immune system in regulating blood sugar. In this experiment mouse skeletal muscle was lysed and run on a gel. FNDC5 was detected at the correct size and gave a nice clean band.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Skeletal Muscle

Primary Incubation

4% BSA in TBST overnight at 4*C

Blocking Agent

5% milk in TBST

Secondary Incubation

5% milk in TBST for 2 hours at room temperature

Tertiary Incubation

N/A

Detection

ECL

Results Summary

In this experiment mouse skeletal muscle was lysed and run on a gel. FNDC5 was detected at the correct size and gave a nice clean band.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great signal detection

The Bad

Abcam antibodies are expensive

The Bottom Line

This antibody gave a nice clean band at the correct size. However, Abcam antibodies are expensive.

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