Great Antibody

September 23, 2019

BlueskyReddit
Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-mTOR (Ser2448) Antibody

Catalog Number:

2971

We are interested in studying the mechanism of T cell differentiation through modulating mTOR pathway.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse T cells from spleen

Primary Incubation

1:2500

Blocking Agent

5% milk

Secondary Incubation

Anti-rabbit HRP 1:5000

Tertiary Incubation

None

Detection

ECL

Results Summary

Works really well to distinguish between high phosphorylated mTOR vs. low.

DOI or PMID #

https://doi.org/10.4049/jimmunol.1701477

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very specific

The Bad

None

The Bottom Line

Very sensitive antibody to detect phosphorylated protein

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