CD169 Antibody for Immunofluorescence

March 08, 2019

Immunology
MSKCC
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

CD169 (Siglec-1) Monoclonal Antibody (SER-4), eFluor 660

Catalog Number:

50-5755-82

Clone Number:

We study tumorgenesis in PyMT model. We would be interested to detect CD169+ cells in tumors. Yet, before that, we stained spleen as a positive control.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed mouse (C57BL/6) spleen cryosections

Primary Incubation

1 in 200

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% rat serum

Secondary Incubation

None

Tertiary Incubation

None

Detection

Images were acquired using an inverted Zeiss LSM880 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Staining of spleen cryosections with B220 (green), CD169 (red) & F4/80 (blue).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable & reproducible staining of spleen

The Bad

None

The Bottom Line

This directly conjugated antibody stains well CD169 cells in the spleen.

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