Clean Isolation of CD4+ T Cells from Splenocytes

University of Pittsburgh
Immunology
Lab Technician

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Company:

Miltenyi Biotec

Product Name:

CD4+ T Cell Isolation Kit, mouse

Catalog Number:

130-104-454

Our lab is studying T Cell signaling during differentiation and what pathways are critical to driving different cell fates. We use this product to isolate CD4+ T Cells from mouse splenocytes in order to perform numerous downstream applications following activation.

Experimental Design and Results Summary

Application

Cell Isolation

Starting Material

Mouse Splenocytes

Protocol Overview

Tips

We follow the provided instructions

Results Summary

We typically get 80-110 million cells from a single spleen and using this kit we are able to isolate a population of approximately 10 million CD4+ T Cells.

DOI or PMID #

10.1074/jbc.RA118.006524

Additional Notes

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Summary

The Good

Easy to use, fast, negative selection

The Bad

Cost

The Bottom Line

This kit has served our needs well for isolation CD4+ T Cells from mouse splenocytes.

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