Nice ELISA

University of Michigan
Pediatrics-Endocrinology
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Raybiotech

Product Name:

Mouse IGF-1 ELISA Kit

Catalog Number:

ELM-IGF1-1

This ELISA kit worked well with our mouse serum samples. It was easy to optimize our mouse pup serum and mouse adult serum samples, in addition to mouse milk samples, diluted 1:1 in PBS

Experimental Design and Results Summary

Application

Measuring Serum IGF-1 Levels

Starting Material

Mouse serum

Protocol Overview

Tips

If you are using skim milk, dilute skimmed milk 1:1 in PBS.

Results Summary

Using Normal Diet C57BL/6J mice.Mom Milk IGF-1 Levels: 100-600 ng/mLNormal Diet Pup Serum IGF-1 Levels: 5-60ng/mLAdult Serum IGF-1 Levels: 10-40 ng/mLHFD Pup Serum IGF-1 Levels: 10-80 ng/mL

DOI or PMID #

N/A

Additional Notes

Image Gallery

Summary

The Good

Dilutions are not required.

The Bad

Some steps are light sensitive and tin foil must be used.

The Bottom Line

It is a reliable, easy-to-use ELISA kit, with a few added complications compared to a Total Insulin ELISA.

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