Good Antibody for Endothelial FACS

March 07, 2019

Biomedical Engineering
UNC Chapel Hill
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

APC anti-human CD31 Antibody

Catalog Number:

303115

Clone Number:

I used this antibody to isolate human intestinal endothelial cells from whole tissue and it worked pretty well. I got ~5% CD31+ cells using this product.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human

Primary Incubation

1:500, 1hr

Blocking Agent

n/a

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

Fluorescence

Results Summary

We isolated CD31+ cells from a mixed solution containing many cell types (epithelium, immune cells, endothelium, etc.).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Worked well with the other antibodies

The Bad

None

The Bottom Line

I trust the antibody to mark the appropriate cells.

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