Abcam
Rabbit anti-GAD 65/67
ab49832
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Browse Similar Products: GAD65 + GAD67 Immunofluorescence Antibodies
I have tried many GAD 65, GAD 67 and combination antibodies to try to label inhibitory interneurons in perfused mouse brain tissue. This antibody is by no means the best but it does the job and provides sufficient staining for colocalization studies where I have used a second antibody.
Immunofluorescence
Mouse brain tissue interneurons
Slices were then quenched with 50 mM ammonium chloride for 15 minutes and washed with PBS
Blocked with a 5% Normal Goat Serum and 1% Bovine Serum Albumin in 0.1M PBS solution
42.4 micrograms/mL for 24 hrs in 0.1 M PBS containing 1% NGS, 0.5% BSA, and 0.1% Tween-20 solution at 4 degrees
Donkey anti-rabbit Alexa 647 of 4µg/ml in 0.1M PBS for 1 h at room temperature
Fluorescence
The GAD 65/67 antibody is used to stain all interneurons in mouse brain slices. The stain is dependent on the quality of the perfusion as poor perfusion can lead to non-specific staining of certain structures. Overall, when combined with a second colocalizing stain this will provide some confirmation on whether the neuron stained is an interneuron. Shown image is at 60x using a confocal microscope.
N/A
Works better than most in the market
Dependent on the perfusion
An OK antibody that is best used in combination with a second stain.