Nice Antibody for Actin

April 01, 2019

Cell Biology
Harvard Medical School
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz

Product Name:

Beta-Actin antibody (C4)

Catalog Number:

sc-47778

This monoclonal beta-actin antibody works very well. I always use it to probe for beta-actin as loading control.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate

Primary Incubation

1:2000, 4℃, overnight or room temperature for 1 hour

Blocking Agent

5% milk

Secondary Incubation

1:5000, room temperature, 1 hour

Tertiary Incubation

None

Detection

ECL

Results Summary

Specific bands were detected at the correct size. The background is usually pretty clean.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Cheap, high specificity and sensitivity

The Bad

None

The Bottom Line

This is one of the best actin antibodies.

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