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Alexa Fluor 700 anti-mouse CD3 Antibody
100216
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In our studies we are interested in T cells and their function and involvement in viral infection clearance. We do the majority of our assays with T cells, so we need a reliable CD3 marker so we know we have selected the correct population for all our analysis. We chose this antibody because it separates well and isn't too overwhelmingly bright.
Flow Cytometry
Mouse mLN cells
Cells were stained in 100ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS
None
LSR II
This antibody shows great separation and shows a clear CD3+ population in our mouse single cell suspension.
N/A
Stable
This is a reliable antibody to use for CD3+ staining in mouse cells.