Bright CD8 for Mouse Cells

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

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Company:

BioLegend

Product Name:

Anti-mouse CD8 BV510

Catalog Number:

100752

Our lab is interested in the function of different T cells before and after viral infection. We need to have a reliable CD8 marker to differentiate CD8 cells from the other cells in our single cell suspension. In order to analyze downstream characteristics of CD8 cells we use this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse mLN cells

Primary Incubation

Cells were stained in 100ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

This antibody separates well and allows us to identify our CD8+ population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright

The Bad

None

The Bottom Line

This is a bright and stable CD8 antibody.

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