Detects Doublet Band at the Correct Molecular Weight

March 25, 2019

Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech

Product Name:

KEAP1 Antibody

Catalog Number:

10503-2-AP

Clone Number:

Antibody used to test lysates from breast cancer cells. A doublet band was detected at the correct size but was not verified with genetic constructs.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Breast Cancer Cells (human)

Primary Incubation

1:1000

Blocking Agent

5% milk

Secondary Incubation

Anti-rabbit IRDye 800

Tertiary Incubation

None

Detection

Licor

Results Summary

A doublet band was detected at the correct size but was not verified with genetic constructs. This was the only band detected in these lysates.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Appears to be working, specific

The Bad

Not a very robust stain

The Bottom Line

This is a usable antibody.

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