Excellent Antibody for Mouse CD25 Staining

March 27, 2019

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

FITC anti-mouse CD25 Antibody

Catalog Number:

102005

Clone Number:

CD25 is a marker for T-cell activation. I used this antibody to measure CD25 expression in resting and TCR-activated T-cells with anti-CD3/CD28 stimulation. This antibody showed quality results with distinct populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T-cells

Primary Incubation

1:200 for 3 hr at 4°C

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody was sensitive enough to differentiate TCR-activated regulatory T-cells and Teff cells when used in combination with Foxp3. I got clear distinct populations.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Ease of optimization and cheap and reproducibility

The Bad

Nothing noted

The Bottom Line

It's worth buying this antibody for reproducibility and ease of optimization.

Share your experience with other scientists. Write a Review! »

Join the discussion