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Alexa Fluor® 700 anti-human CD3 Antibody
300424
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This antibody has been used to optimise flow cytometry-based immunophenotyping of whole-blood samples. The results have not yet been published. Multi-colour antibody panels were designed to identify a number of lymphocyte populations and tested to ensure appropriate compensation was possible. CD3 was used in a ten-colour panel to help identify T cells. It is easy to use and works well.
Flow Cytometry
Human whole blood
2µL AF700-CD3 for 30 minutes at 4°C
FcR blocking reagent, human (Miltenyi Biotec, cat# 130-059-901)
N/A
Beckman Coulter Gallios, Kaluza Software
Staining allowed the distinction of CD3+ cells. Titration of the antibody indicated that optimal staining was achieved at the manufacturers suggested dilution. This antibody fit well into a ten-colour panel with no compensation issues.
Clear staining of CD3+ cells that is useful for FACS analysis.
The antibody has only been tested for flow cytometry.
Easy-to-use antibody pack that provided distinct staining of T cells.