Abcam
Anti-Iba1 antibody [EPR16588]
ab178846
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The primary goal of our study is to investigate inflammatory responses in neurodegenerative diseases. This experiment was sought to evaluate microglial activation in a large animal model. This antibody provided specific and robust staining for optic nerve microglia.
Immunofluorescence
Feline optic nerve tissues
Diluted at 1:2000, overnight at 4°C
10% normal donkey serum + 0.5% triton X + 0.1M PBS
Alexa 568 conjugated anti-rabbit IgG 1:500, 1 hr at RT
N/A
Fluorescence
The IBA1 antibody stains microglia in feline optic nerve tissues without background. As this antibody is a rabbit monoclonal antibody, the specificity is better than that of polyclonal antibodies.
Specific monoclonal rabbit antibody for microglia
None
Highly recommended antibody for immunofluorescence of feline microglia on tissue sections.