BD Biosciences
PE Mouse Anti-Human CD45RA
555489
Browse Similar Products: CD45RA Flow Cytometry Antibodies
The identification of naïve T cells relies on the correct characterization of T cells (using CD3), and then looking at their subsets (helper T cells: CD4+, and cytotoxic T cells: CD8+). These cells before they encounter an antigen (naïve T cells) will also express CD45RA. After antigen experience, central and effector memory T cells gain expression of CD45RO and lose expression of CD45RA. In our lab we are characterizing these subsets and so chose this antibody to go with the CD3/CD4/CD8/CD45RO panel we devised.
Flow Cytometry
Peripheral Blood
15 minutes at 4 degrees on ice
BSA (1%)
N/A
In FL-2 channel (blue laser) flow cytometer
The majority of the lymphocytes shown in the plot (blue) stained positive with this antibody; however, we needed to do some optimization to get the signal right. Being so bright causes issues, especially with compensation.The plot on the left is the negative control.
Affordable. Bright fluorescence
Too bright! Compensation issues inevitable
It's a good antibody, but you have to be careful with compensation, and it is imperative to titrate it.