Anti-Human CD56 Antibody BUV737

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BUV737 Mouse Anti-Human CD56

Catalog Number:

564448

Clone Number:

Browse Similar Products: CD56 Flow Cytometry Antibodies

The CD56 antigen is expressed on approximately 10% to 25% of peripheral blood lymphocytes. It is present on essentially all resting and activated CD16+ natural killer (NK) lymphocytes and approximately 5% of CD3+ peripheral blood lymphocytes, as well as on a fraction of ILC3s, at least in the tonsil, the subset of cells we are interested in.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

15 min, 37°c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD FACSymphony, software FACS Diva

Results Summary

Bright antibody with high resolution, enables the identification of both CD56bright and CD56dim NK cells. BUV737 has some spill over into other channels such as AF700, as seen in the graph, but it is workable.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright, high resolution

The Bad

Some spill over into AF700

The Bottom Line

Good antibody, would recommend

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