Anti-Human T-bet PECy7

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Cy7 anti-T-bet Antibody

Catalog Number:

644823

Clone Number:

T-bet is a master regulator of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, T cells, and group 1 innate lymphoid cells, which we are interested in.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

15 min, 37°c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD FACSsymphony, software FCS Diva

Results Summary

Good, specific staining on T cells, NK cells and ILC1s. Antibody is extremely concentrated and was diluted to 1:800. Resolution is decent.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Specific, good resolution

The Bad

Extremely concentrated, must be diluted upwards of 1:800

The Bottom Line

Good antibody, would recommend

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