PE/Cy7 anti-T-bet Antibody
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T-bet is a master regulator of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, T cells, and group 1 innate lymphoid cells, which we are interested in.
15 min, 37°c
0.5% BSA in PBS
BD FACSsymphony, software FCS Diva
Good, specific staining on T cells, NK cells and ILC1s. Antibody is extremely concentrated and was diluted to 1:800. Resolution is decent.
Specific, good resolution
Extremely concentrated, must be diluted upwards of 1:800
Good antibody, would recommend