Anti-Human CRTH2 Antibody

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cyanine5.5 anti-human CD294 (CRTH2) Antibody

Catalog Number:

350116

Clone Number:

CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. Most recently described to be expressed by group 2 innate lymphoid cells

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Flow Cytometry

Primary Incubation

15 min, 37°c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD FACSymphony, FACSdiva

Results Summary

CRTH2 is notoriously difficult to obtain a great staining from. This antibody is functional and stains CRTH2, but it remains very dim and has low resolution. Other inclusion markers are required for a clear view of CRTH2 on a specific population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific

The Bad

Low resolution

The Bottom Line

Okay antibody for CRTH2

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