Anti-Human CD3 Antibody

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BUV805 Mouse Anti-Human CD3

Catalog Number:

565511

Clone Number:

Browse Similar Products: CD3 Flow Cytometry Antibodies

CD3 is part of the TCR signaling complex and is present on T lymphocytes as found in peripheral blood. We use CD3 outside of lineage/dump channels to securely identify and exclude T cells from our panels, where our main interest are innate lymphoid cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

15 min, 37°c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD FACSymphony, software FACSDiva

Results Summary

This antibody gives a bright signal and is specific for CD3-expressing T cells. High resolution.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific, bright, and high resolution

The Bad

Some spillover into BUV737, use mutually exclusive markers in these channels

The Bottom Line

Works well, would recommend.

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