Anti-Human CD127 BUV395

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BUV395 Mouse Anti-Human CD127

Catalog Number:

742547

Clone Number:

Browse Similar Products: CD127 Flow Cytometry Antibodies

CD127 (IL-7Rα) forms a heterodimer with the common gamma chain and is expressed on thymocytes, T- and B-cell progenitors, mature T cells, and some lymphoid and myeloid cells. We look at expression of CD127 in various subsets of innate cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

15 min, 37°c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD FACSymphony, software FACS Diva

Results Summary

This anti-human CD127 antibody is extremely bright and has good resolution, enabling the resolution of high and low expression on non-T-cells. BUV395 is a bright fluorochrome that does not have significant spillage onto other channels.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright, high resolution

The Bad

N/A

The Bottom Line

Works nicely, would recommend

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