Cell Signaling Technology
Rheb (E1G1R) Rabbit mAb
13879
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My research focus is on understanding the role of the immune system in regulating blood glucose and adipose tissue. For this experiment, mouse CD4+ T-cells were stimulated or stimulated with rapamycin. Our lab is interested in mTOR signaling so we probed for Rheb protein levels.
Western Blot
CD4+ mouse T-cells
4% BSA in TBST overnight at 4°C
5% milk in TBST
5% milk in TBST for 2 hours at room temperature
N/A
ECL
Mouse CD4+ T-cells were non-stimulated (left 4), stimulated (middle 4) or stimulated with rapamycin (right 4). Rheb total protein levels were interrogated.
Great signal detection
Expensive antibody
The antibody works very well. You just need to be careful that you don't run Rheb off of your gel.